We propose that BRP is critical for intact AZ structure and normal-evoked neurotransmitter release at chemical synapses of Drosophila. In adults, we observe loss of T bars at active zones, absence of synaptic components in electroretinogram, locomotor inactivity, and unstable flight (hence “ bruchpilot”-crash pilot). Panneural reduction of BRP expression by RNAi constructs permits a first functional characterization of this large AZ protein: larvae show reduced evoked but normal spontaneous transmission at neuromuscular junctions. During development, transcription of the bruchpilot locus ( brp) coincides with neuronal differentiation. The C terminus of BRP displays structural similarities to multifunctional cytoskeletal proteins. BRP shows homology to human AZ protein ELKS/CAST/ERC, which binds RIM1 in a complex with Bassoon and Munc13-1. Display version 2.0 or 2.1 will be released soon also. 1.17 ) before installing the display firmware (vers. We employ nc82 to identify Bruchpilot protein (BRP) as a previously unknown AZ component. Here is a link to the firmware- Once you get the USB in the display you will need to make sure to install the Monitor Firmware (vers. In the fly Drosophila, monoclonal antibody (MAB) nc82 specifically labels AZs. Neurotransmitters are released at presynaptic active zones (AZs).
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